The thesis for Ph. D. Degree (Biology) on the specialty 03.00.16 – ecology. – Taras Shevchenko Kyiv National University, 2002.
A research of virus spectrum in the reserve of “Askania–Nova”, which is accented not on the cultural plants but on the wild ones, has been conducted. It gives the possibility to establish the complicated “host–parasite” relations with minimum influence of anthropogenic factor.
It was shown that the viral diversity depends on the floristical spectrum and anthropogenic load. The territories of dendropark and the steppe areas bordering agrocenose, the territories of dendropark with steppe and agrocenose, the areas bordering dendropark and Chapel lowland are the richest in the number of viruses.
The isolates of wheat srtik mosaic virus, tobacco mosaic virus and cucumber mosaic virus with different peculiarities (point of temperature inactivation, maximum juice dilution, degree of influence on photosynthetic apparatus of plant cells) are educed from wild plants. The mentioned above characteristics of isolates correlated with peculiarities of the microclimate of their habitat.
The chlorophyll fluorescence induction method has been applied in order to study the pathogenicity of the educed isolates. Quantitative estimation of impact virus infection on the activity of photosynthetic apparatus by means of fluorescence analysis allowed us to detect the differences in the pathogenic action of the isolates, which cannot be register visually.
The reproduction of a pathogene in new ecological niche was investigated using clinostating for simulation of zero gravity conditions. It has been determined that during the clinostating the activity of photosynthetic apparatus and other physiological processes was reduced. Consequently, it resulted in the decreasing of reproduction and accumulation of wheat stik mosaic virus affecting concentration of virus particles in leaf juice.
Key words: phytovirus, biocenose, fluorescence induction, clinostating.